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there are actually tyrosine (Y223) and lysine (K139) residues that can also be contributing for the interaction With all the phosphate moiety of the substrate. Deprotonation of possibly of such residues at substantial pH could bring about a drop in the worth of k
The 2nd ionization observed in the log kcat/Km profile could originate from other residues in the Lively site of NagA nonetheless it could also come up from the protonation with the substrate by itself since the phosphate moiety features a p
[72] The actions on the bakunawa served as being a geomantic calendar process for historic Filipinos and had been Element of the shamanistic rituals of the babaylan. It will likely be depicted using a characteristically looped tail and was variously thought to inhabit possibly The ocean, the sky, or maybe the underworld.[seventy three]
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This enlargement during the "Naga" identification has long been due to a number of factors such as the quest for upward mobility in the Culture of Nagaland, and the need to establish a standard objective of resistance versus dominance by other teams. In this manner, the "Naga" identification has not often been mounted.[twenty]
While wars, nature, plus the passage of your time destroyed many temples within the Funan era, nāgas can even now be viewed in ancient temples dating to the Chenla and Angkor eras.
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It's also a foundation medical center of the Helen Keller Foundation, the place eye experts from all over the nation are trained and later on assigned to distinct aspects of the Philippines.[sixty one]
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While in the crystal construction with the D273N mutant of NagA, the side chain of His-143 is 3.0 Å away from the phosphonate oxygen with the mimic from the tetrahedral intermediate (21). This consequence suggests that this team could facilitate the activation on the carbonyl team in the substrate at the side of an conversation Using the steel ion bound in the active internet site.
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